PDBe 5eu6

X-ray diffraction
2.02Å resolution

HLA Class I antigen

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
V_segment translation product; T-cell receptor beta-2 chain C region Chain: E
Molecule details ›
Chain: E
Length: 244 amino acids
Theoretical weight: 27.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A5B9 (Residues: 1-129; Coverage: 73%)
  • Canonical: A0A592 (Residues: 20-115; Coverage: 99%)
  • nullnull
Gene names: TCRBC2, TCRBV6S1A1N1, TRBC2
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
T cell receptor alpha variable 21; T-cell receptor alpha chain C region Chain: D
Molecule details ›
Chain: D
Length: 204 amino acids
Theoretical weight: 22.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01848 (Residues: 1-89; Coverage: 63%)
  • Canonical: A0A0B4J279 (Residues: 20-111; Coverage: 99%)
  • nullnull
Gene names: TCRA, TRAC, TRAV21
Structure domains: Immunoglobulins
M-alpha Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 974 Da
Source organism: synthetic construct
Expression system: synthetic construct
UniProt:
  • Canonical: P40967 (Residues: 280-287; Coverage: 1%)
Gene names: D12S53E, PMEL, PMEL17, SILV
HLA class I histocompatibility antigen, A-2 alpha chain Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 31.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01892 (Residues: 25-300; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P1
Unit cell:
a: 45.52Å b: 54.41Å c: 112.12Å
α: 85.01° β: 81.64° γ: 72.63°
R-values:
R R work R free
0.183 0.181 0.222
Expression systems:
  • Escherichia coli
  • synthetic construct