PDBe 5eu4

X-ray diffraction
2.12Å resolution

HLA Class I antigen

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Beta-2-microglobulin Chains: B, E
Molecule details ›
Chains: B, E
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
M-alpha Chains: C, F
Molecule details ›
Chains: C, F
Length: 9 amino acids
Theoretical weight: 888 Da
Source organism: synthetic construct
Expression system: synthetic construct
UniProt:
  • Canonical: P40967 (Residues: 280-288; Coverage: 1%)
Gene names: D12S53E, PMEL, PMEL17, SILV
HLA class I histocompatibility antigen, A-2 alpha chain Chains: A, D
Molecule details ›
Chains: A, D
Length: 276 amino acids
Theoretical weight: 31.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01892 (Residues: 25-300; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P1
Unit cell:
a: 56.08Å b: 57.63Å c: 79.93Å
α: 90° β: 89.81° γ: 63.84°
R-values:
R R work R free
0.174 0.172 0.211
Expression systems:
  • Escherichia coli
  • synthetic construct