PDBe 5eoa

X-ray diffraction
2.5Å resolution

Crystal structure of OPTN E50K mutant and TBK1 complex

Released:

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Optineurin Chains: A, B
Molecule details ›
Chains: A, B
Length: 82 amino acids
Theoretical weight: 9.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96CV9 (Residues: 26-103; Coverage: 14%)
  • Best match: Q96CV9-3 (Residues: 1-46)
Gene names: FIP2, GLC1E, HIP7, HYPL, NRP, OPTN
Serine/threonine-protein kinase TBK1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 57 amino acids
Theoretical weight: 6.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UHD2 (Residues: 677-729; Coverage: 7%)
Gene names: NAK, TBK1

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 36.824Å b: 54.549Å c: 153.305Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.199 0.267
Expression system: Escherichia coli