PDBe 5ecg

X-ray diffraction
3Å resolution

Crystal structure of the BRCT domains of 53BP1 in complex with p53 and H2AX-pSer139 (gammaH2AX)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Cellular tumor antigen p53 Chains: A, B
Molecule details ›
Chains: A, B
Length: 225 amino acids
Theoretical weight: 25.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04637 (Residues: 95-312; Coverage: 56%)
  • Best match: P04637-9 (Residues: 1-180)
Gene names: P53, TP53
Sequence domains: P53 DNA-binding domain
Structure domains: Immunoglobulin-like
TP53-binding protein 1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 262 amino acids
Theoretical weight: 29.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q12888 (Residues: 1713-1972; Coverage: 13%)
Gene name: TP53BP1
Sequence domains: BRCA1 C Terminus (BRCT) domain
Structure domains: BRCT domain
SEP-GLN-GLU-TYR Chains: E, F
Molecule details ›
Chains: E, F
Length: 4 amino acids
Theoretical weight: 605 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 70.264Å b: 94.498Å c: 131.742Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.204 0.264
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided