PDBe 5e9d

X-ray diffraction
2.51Å resolution

RD-1 Mart-1 High bound to Mart-1 decameric peptide (ELA) in complex with HLA-A2

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
A6-TCR Vbeta Chains: E, J
Molecule details ›
Chains: E, J
Length: 156 amino acids
Theoretical weight: 16.92 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
A6-TCR Valpha Chains: D, I
Molecule details ›
Chains: D, I
Length: 129 amino acids
Theoretical weight: 14.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Beta-2-microglobulin Chains: B, G
Molecule details ›
Chains: B, G
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Melanoma antigen recognized by T-cells 1 Chains: C, H
Molecule details ›
Chains: C, H
Length: 10 amino acids
Theoretical weight: 985 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q16655 (Residues: 26-35; Coverage: 9%)
Gene names: MART1, MLANA
HLA class I histocompatibility antigen, A-2 alpha chain Chains: A, F
Molecule details ›
Chains: A, F
Length: 275 amino acids
Theoretical weight: 31.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01892 (Residues: 25-299; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 50.438Å b: 164.484Å c: 95.201Å
α: 90° β: 100.56° γ: 90°
R-values:
R R work R free
0.183 0.18 0.232
Expression systems:
  • Escherichia coli
  • Not provided