PDBe 5e6i

X-ray diffraction
4Å resolution

Crystal structure of TCR PF8 in complex with flu MP(58-66) epitope presented by HLA-A2

Released:
Entry authors: Gao M, Mariuzza R

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero heptamer
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
TCR alpha chain,Human nkt tcr alpha chain Chains: A, F, G, P
Molecule details ›
Chains: A, F, G, P
Length: 208 amino acids
Theoretical weight: 22.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins
T-cell receptor beta-2 chain C region Chains: B, H, L, Q
Molecule details ›
Chains: B, H, L, Q
Length: 243 amino acids
Theoretical weight: 27.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins
HLA class I histocompatibility antigen, A-2 alpha chain Chains: C, I, M, R
Molecule details ›
Chains: C, I, M, R
Length: 276 amino acids
Theoretical weight: 31.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: NEW P01892 (Residues: 25-299; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: D, J, N, U
Molecule details ›
Chains: D, J, N, U
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: NEW P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Matrix protein 1 Chains: E, K, O, T
Molecule details ›
Chains: E, K, O, T
Length: 9 amino acids
Theoretical weight: 966 Da
Source organism: Influenza A virus (A/Boston/2747243M/2009(H1N1))
Expression system: Not provided
UniProt:
  • Canonical: NEW P03485 (Residues: 58-66; Coverage: 4%)
Gene name: M

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P1
Unit cell:
a: 71.853Å b: 123.614Å c: 139.036Å
α: 83.66° β: 74.87° γ: 73.17°
R-values:
R R work R free
0.263 0.259 0.338
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided