PDBe 5ds5

X-ray diffraction
2.95Å resolution

Crystal structure the Escherichia coli Cas1-Cas2 complex bound to protospacer DNA and Mg

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
CRISPR-associated endonuclease Cas1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 306 amino acids
Theoretical weight: 33.32 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: Q46896 (Residues: 1-305; Coverage: 100%)
Gene names: JW2725, b2755, cas1, ygbT
Sequence domains: CRISPR associated protein Cas1
Structure domains:
CRISPR-associated endoribonuclease Cas2 Chains: E, F
Molecule details ›
Chains: E, F
Length: 104 amino acids
Theoretical weight: 11.68 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P45956 (Residues: 1-94; Coverage: 100%)
Gene names: JW5438, b2754, cas2, ygbF
Sequence domains: CRISPR-associated protein (Cas_Cas2CT1978)
DNA (28-MER) Chain: G
Molecule details ›
Chain: G
Length: 28 nucleotides
Theoretical weight: 8.68 KDa
DNA (28-MER) Chain: H
Molecule details ›
Chain: H
Length: 28 nucleotides
Theoretical weight: 8.53 KDa

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 75.656Å b: 165.928Å c: 167.258Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.234 0.256
Expression system: Escherichia coli