› 5dmi

X-ray diffraction
3.69Å resolution

Structure of the extracellular domain of the CD40 in complex with CHI220 FAB

Released:
DOI: 10.2210/pdb5dmi/pdb
PDB entry 5dmi coloured by chain, front view.
PDB entry 5dmi coloured by chain, side view.
PDB entry 5dmi coloured by chain, top view.
Source organisms:
Primary publication:
Functional Antagonism of Human CD40 Achieved by Targeting a Unique Species-Specific Epitope.
Yamniuk AP, Suri A, Krystek SR, Tamura J, Ramamurthy V, Kuhn R, Carroll K, Fleener C, Ryseck R, Cheng L, An Y, Drew P, Grant S, Suchard SJ, Nadler SG, Bryson JW, Sheriff S
J Mol Biol 428 2860-79 (2016)
PMID: 27216500

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-229926 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tumor necrosis factor receptor superfamily member 5 Chain: A
Molecule details ›
Chain: A
Length: 183 amino acids
Theoretical weight: 20.08 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P25942 (Residues: 23-193; Coverage: 67%)
Gene names: CD40, TNFRSF5
Sequence domains: TNFR/NGFR cysteine-rich region
Chi220 Fab heavy chain Chain: H
Molecule details ›
Chain: H
Length: 223 amino acids
Theoretical weight: 23.91 KDa
Source organism: Mus musculus
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
Chi220 Fab light chain Chain: L
Molecule details ›
Chain: L
Length: 213 amino acids
Theoretical weight: 23.36 KDa
Source organism: Mus musculus
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P42212
Unit cell:
a: 201.152Å b: 201.152Å c: 63.809Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.168 0.207
Expression systems:
  • Homo sapiens
  • Cricetulus griseus

Quick links

Citations

4 review citations

OX40: Structure and function - What questions remain?
Willoughby et al. (2017)
3 more

1 mention without citation

Solvent Gaming Chemistry to Control the Quality of Halide Perovskite Thin Films for Photovoltaics.
Huang et al. (2022)