PDBe 5dmg

X-ray diffraction
2.5Å resolution

X-RAY STRUCTURE OF THE FAB FRAGMENT OF THE ANTI TAU ANTIBODY RB86 IN COMPLEX WITH THE PHOSPHORYLATED TAU PEPTIDE (416-430)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
RB86 antibody Fab fragment heavy chain Chains: C, E, H
Molecule details ›
Chains: C, E, H
Length: 211 amino acids
Theoretical weight: 21.98 KDa
Source organism: Oryctolagus cuniculus
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
RB86 antibody Fab fragment light chain Chains: D, F, L
Molecule details ›
Chains: D, F, L
Length: 219 amino acids
Theoretical weight: 23.54 KDa
Source organism: Oryctolagus cuniculus
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
Microtubule-associated protein tau Chains: P, X, Z
Molecule details ›
Chains: P, X, Z
Length: 15 amino acids
Theoretical weight: 1.66 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P10636 (Residues: 733-747; Coverage: 2%)
Gene names: MAPT, MAPTL, MTBT1, TAU

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 67.51Å b: 163.053Å c: 70.926Å
α: 90° β: 110.27° γ: 90°
R-values:
R R work R free
0.212 0.21 0.263
Expression systems:
  • Cricetulus griseus
  • Not provided