› 5dgx

X-ray diffraction
1.73Å resolution

1.73 Angstrom resolution crystal structure of the ABC-ATPase domain (residues 357-609) of lipid A transport protein (msbA) from Francisella tularensis subsp. tularensis SCHU S4 in complex with ADP

Released:
DOI: 10.2210/pdb5dgx/pdb
PDB entry 5dgx coloured by chain, front view.
PDB entry 5dgx coloured by chain, side view.
PDB entry 5dgx coloured by chain, top view.
Source organism: Francisella tularensis subsp. tularensis FSC198
Entry authors: Halavaty AS, Minasov G, Dubrovska I, Kiryukhina O, Grimshaw S, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + lipid A-core oligosaccharide(Side 1) = ADP + phosphate + lipid A-core oligosaccharide(Side 2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172146 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent lipid A-core flippase Chain: A
Molecule details ›
Chain: A
Length: 256 amino acids
Theoretical weight: 27.84 KDa
Source organism: Francisella tularensis subsp. tularensis FSC198
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14JW6 (Residues: 357-609; Coverage: 42%)
Gene names: FTF0109, msbA
Sequence domains: ABC transporter
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21
Unit cell:
a: 39.597Å b: 70.886Å c: 46.647Å
α: 90° β: 103.77° γ: 90°
R-values:
R R work R free
0.196 0.194 0.233
Expression system: Escherichia coli

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