PDBe 5dc9

X-ray diffraction
1.56Å resolution

CRYSTAL STRUCTURE OF MONOBODY AS25/ABL1 SH2 DOMAIN COMPLEX, CRYSTAL B

Released:

Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosine-protein kinase ABL1 Chain: A
Molecule details ›
Chain: A
Length: 123 amino acids
Theoretical weight: 13.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00519 (Residues: 112-232; Coverage: 11%)
Gene names: ABL, ABL1, JTK7
Sequence domains: SH2 domain
Structure domains: SH2 domain
Ugl-Y3 Chain: B
Molecule details ›
Chain: B
Length: 95 amino acids
Theoretical weight: 10.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P02751 (Residues: 1445-1540; Coverage: 4%)
Gene names: FN, FN1
Sequence domains: Fibronectin type III domain
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P41212
Unit cell:
a: 66.391Å b: 66.391Å c: 112.797Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.138 0.137 0.171
Expression system: Escherichia coli BL21(DE3)