PDBe 5d7q

X-ray diffraction
2.01Å resolution

Crystal structure of human Sirt2 in complex with ADPR and CHIC35

Released:
Source organism: Homo sapiens
Primary publication:
Seeding for sirtuins: microseed matrix seeding to obtain crystals of human Sirt3 and Sirt2 suitable for soaking.
OpenAccess logo Acta Crystallogr F Struct Biol Commun 71 1498-510 (2015)
PMID: 26625292

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD-dependent protein deacetylase sirtuin-2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 304 amino acids
Theoretical weight: 34.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8IXJ6 (Residues: 56-356; Coverage: 77%)
Gene names: SIR2L, SIR2L2, SIRT2
Sequence domains: Sir2 family

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 77.34Å b: 78.17Å c: 114.68Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.211 0.225
Expression system: Escherichia coli