PDBe 5d4b

X-ray diffraction
2.66Å resolution

Structural Basis for a New Templated Activity by Terminal Deoxynucleotidyl Transferase: Implications for V(D)J Recombination

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA nucleotidylexotransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 400 amino acids
Theoretical weight: 45.7 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P09838 (Residues: 132-510; Coverage: 74%)
Gene names: Dntt, Tdt
Sequence domains:
Structure domains:
DNA (5'-D(*AP*AP*AP*AP*AP*C)-3') Chains: C, D, E
Molecule details ›
Chains: C, D, E
Length: 6 nucleotides
Theoretical weight: 1.81 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*TP*TP*TP*TP*TP*GP*G)-3') Chain: F
Molecule details ›
Chain: F
Length: 7 nucleotides
Theoretical weight: 2.13 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P212121
Unit cell:
a: 56.73Å b: 71.45Å c: 198.17Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.207 0.247
Expression systems:
  • Escherichia coli
  • Not provided