PDBe 5d3o

X-ray diffraction
2.79Å resolution

Crystal structure of full length human glutaminase C expressed in E.coli

Released:
Source organism: Homo sapiens
Entry author: Huang Q

Function and Biology Details

Reaction catalysed:
L-glutamine + H(2)O = L-glutamate + NH(3)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutaminase kidney isoform, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 527 amino acids
Theoretical weight: 58.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O94925 (Residues: 72-550; Coverage: 72%)
  • Best match: O94925-2 (Residues: 72-161)
Gene names: GLS, GLS1, KIAA0838
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Unit cell:
a: 51.609Å b: 139.371Å c: 179.789Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.214 0.266
Expression system: Escherichia coli BL21(DE3)