PDBe 5d3f

X-ray diffraction
2.74Å resolution

Crystal structure of human 14-3-3 zeta in complex with CFTR R-domain peptide pS753-pS768 and stabilizer fusicoccin-A


Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein zeta/delta Chains: A, B
Molecule details ›
Chains: A, B
Length: 230 amino acids
Theoretical weight: 26.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: P63104 (Residues: 1-230; Coverage: 94%)
Gene name: YWHAZ
Sequence domains: 14-3-3 protein
Structure domains: 14-3-3 domain
Cystic fibrosis transmembrane conductance regulator Chain: C
Molecule details ›
Chain: C
Length: 28 amino acids
Theoretical weight: 3.26 KDa
Source organism: Homo sapiens
Expression system: Not provided
  • Canonical: P13569 (Residues: 747-774; Coverage: 2%)
Gene names: ABCC7, CFTR

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: C2221
Unit cell:
a: 69.607Å b: 110.166Å c: 132.604Å
α: 90° β: 90° γ: 90°
R R work R free
0.248 0.246 0.303
Expression systems:
  • Escherichia coli
  • Not provided