PDBe 5d39

X-ray diffraction
3.2Å resolution

Transcription factor-DNA complex

Released:
Source organism: Homo sapiens
Primary publication:
Structural basis for DNA recognition by STAT6.
Proc. Natl. Acad. Sci. U.S.A. 113 13015-13020 (2016)
PMID: 27803324

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero octamer
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Signal transducer and activator of transcription 6 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 539 amino acids
Theoretical weight: 60.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P42226 (Residues: 123-658; Coverage: 63%)
Gene name: STAT6
Sequence domains:
DNA (5'-D(P*AP*TP*GP*GP*AP*TP*TP*TP*CP*CP*TP*GP*GP*AP*AP*GP*AP*CP*AP*GP*A)-3') Chains: E, M
Molecule details ›
Chains: E, M
Length: 21 nucleotides
Theoretical weight: 6.51 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(P*TP*CP*TP*GP*TP*CP*TP*TP*CP*CP*AP*GP*GP*AP*AP*AP*TP*CP*CP*AP*T)-3') Chains: F, N
Molecule details ›
Chains: F, N
Length: 21 nucleotides
Theoretical weight: 6.37 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P1
Unit cell:
a: 68.395Å b: 94.697Å c: 145.642Å
α: 79.62° β: 78.31° γ: 89.58°
R-values:
R R work R free
0.211 0.21 0.236
Expression systems:
  • Escherichia coli BL21
  • Not provided