PDBe 5czi

X-ray diffraction
2.6Å resolution

EGFR L858R MUTANT IN COMPLEX WITH A SHC PEPTIDE SUBSTRATE

Released:

Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Epidermal growth factor receptor Chain: A
Molecule details ›
Chain: A
Length: 331 amino acids
Theoretical weight: 37.68 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P00533 (Residues: 694-1022; Coverage: 28%)
Gene names: EGFR, ERBB, ERBB1, HER1
Sequence domains: Protein tyrosine kinase
Structure domains:
SHC-transforming protein 1 Chain: B
Molecule details ›
Chain: B
Length: 9 amino acids
Theoretical weight: 1.28 KDa
Source organism: Escherichia coli H489
Expression system: Not provided
UniProt:
  • Canonical: P29353 (Residues: 345-353; Coverage: 2%)
Gene names: SHC, SHC1, SHCA

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: I23
Unit cell:
a: 143.89Å b: 143.89Å c: 143.89Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.183 0.225
Expression systems:
  • Spodoptera frugiperda
  • Not provided