PDBe 5cvm

X-ray diffraction
1.9Å resolution

USP46~ubiquitin BEA covalent complex

Released:

Function and Biology Details

Reaction catalysed:
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ubiquitin Chain: B
Molecule details ›
Chain: B
Length: 96 amino acids
Theoretical weight: 10.75 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P0CG47 (Residues: 153-211; Coverage: 26%)
Gene name: UBB
Ubiquitin carboxyl-terminal hydrolase 46 Chain: A
Molecule details ›
Chain: A
Length: 358 amino acids
Theoretical weight: 41.96 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62068 (Residues: 25-366; Coverage: 93%)
  • Best match: P62068-2 (Residues: 1-217)
Gene name: USP46
Sequence domains: Ubiquitin carboxyl-terminal hydrolase
Structure domains: Cysteine proteinases

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: C2221
Unit cell:
a: 90.613Å b: 105.12Å c: 134.656Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.176 0.197
Expression system: Trichoplusia ni