PDBe 5cjo

X-ray diffraction
3.29Å resolution

Crystal Structure Analysis of Elbow-Engineered-Fab-Bound Human Insulin Degrading Enzyme (IDE) in Complex with Insulin

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Insulin-degrading enzyme Chain: A
Molecule details ›
Chain: A
Length: 990 amino acids
Theoretical weight: 114.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P14735 (Residues: 42-1019; Coverage: 96%)
Gene name: IDE
Sequence domains:
Structure domains: Metalloenzyme, LuxS/M16 peptidase-like
FAB Heavy chain with engineered elbow Chain: H
Molecule details ›
Chain: H
Length: 239 amino acids
Theoretical weight: 25.73 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
FAB light chain Chain: L
Molecule details ›
Chain: L
Length: 216 amino acids
Theoretical weight: 23.45 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Insulin A chain Chain: a
Molecule details ›
Chain: a
Length: 20 amino acids
Theoretical weight: 2.27 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01308 (Residues: 90-109; Coverage: 23%)
Gene name: INS

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Unit cell:
a: 75.689Å b: 109.218Å c: 263.405Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.196 0.245
Expression systems:
  • Escherichia coli
  • Not provided