PDBe 5c67

X-ray diffraction
1.83Å resolution

Human Mesotrypsin in complex with amyloid precursor protein inhibitor variant APPI-M17G/I18F/F34V


Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Trypsin-3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 224 amino acids
Theoretical weight: 24.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
  • Canonical: P35030 (Residues: 81-304; Coverage: 74%)
Gene names: PRSS3, PRSS4, TRY3, TRY4
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Soluble APP-beta Chains: C, E
Molecule details ›
Chains: C, E
Length: 81 amino acids
Theoretical weight: 9.13 KDa
Source organism: Homo sapiens
Expression system: Komagataella phaffii GS115
  • Canonical: P05067 (Residues: 294-346; Coverage: 7%)
  • Best match: P05067-7 (Residues: 294-346)
Gene names: A4, AD1, APP
Structure domains: Pancreatic trypsin inhibitor Kunitz domain

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P41212
Unit cell:
a: 78.15Å b: 78.15Å c: 243.73Å
α: 90° β: 90° γ: 90°
R R work R free
0.224 0.223 0.267
Expression systems:
  • Escherichia coli BL21
  • Komagataella phaffii GS115