PDBe 5c4v

X-ray diffraction
2.6Å resolution

Ski-like protein

Released:
Source organism: Homo sapiens
Primary publication:
SnoN Stabilizes the SMAD3/SMAD4 Protein Complex.
OpenAccess logo Sci Rep 7 46370 (2017)
PMID: 28397834

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mothers against decapentaplegic homolog 4 Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 258 amino acids
Theoretical weight: 28.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13485 (Residues: 314-549; Coverage: 43%)
Gene names: DPC4, MADH4, SMAD4
Sequence domains: MH2 domain
Structure domains: Tumour Suppressor Smad4
Ski-like protein Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 127 amino acids
Theoretical weight: 14.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P12757 (Residues: 238-356; Coverage: 17%)
Gene names: SKIL, SNO
Sequence domains: c-SKI Smad4 binding domain
Structure domains: SAND domain-like

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: C2
Unit cell:
a: 213.54Å b: 122.83Å c: 51.57Å
α: 90° β: 90.72° γ: 90°
R-values:
R R work R free
0.209 0.208 0.242
Expression system: Escherichia coli