PDBe 5c13

X-ray diffraction
2.1Å resolution

Crystal Structure of TAF3 PHD finger bound to histone H3C4me3 peptide

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Transcription initiation factor TFIID subunit 3 Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 64 amino acids
Theoretical weight: 7.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: NEW Q5VWG9 (Residues: 855-915; Coverage: 7%)
Gene name: TAF3
Sequence domains: PHD-finger
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)
Histone H3.1 Chains: D, F, H, P
Molecule details ›
Chains: D, F, H, P
Length: 10 amino acids
Theoretical weight: 1.19 KDa
Source organism: synthetic construct
Expression system: Not provided
UniProt:
  • Canonical: NEW P68431 (Residues: 2-11; Coverage: 7%)
Gene names: H3FA, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 30.212Å b: 50.105Å c: 85.949Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.222 0.28
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided