PDBe 5btr

X-ray diffraction
3.2Å resolution

Crystal structure of SIRT1 in complex with resveratrol and an AMC-containing peptide

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
SirtT1 75 kDa fragment Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 397 amino acids
Theoretical weight: 45.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96EB6 (Residues: 143-513, 514-515; Coverage: 50%)
Gene names: SIR2L1, SIRT1
Sequence domains: Sir2 family
Structure domains:
AMC-containing peptide Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 5 amino acids
Theoretical weight: 796 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P41
Unit cell:
a: 133.89Å b: 133.89Å c: 106.707Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.207 0.252
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided