PDBe 5brr

X-ray diffraction
3.16Å resolution

Michaelis complex of tPA-S195A:PAI-1

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Plasminogen activator inhibitor 1 Chain: I
Molecule details ›
Chain: I
Length: 379 amino acids
Theoretical weight: 42.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P05121 (Residues: 24-402; Coverage: 100%)
Gene names: PAI1, PLANH1, SERPINE1
Sequence domains: Serpin (serine protease inhibitor)
Structure domains:
Tissue-type plasminogen activator chain B Chain: E
Molecule details ›
Chain: E
Length: 252 amino acids
Theoretical weight: 28.12 KDa
Source organism: Homo sapiens
Expression system: Komagataella phaffii CBS 7435
UniProt:
  • Canonical: P00750 (Residues: 311-562; Coverage: 47%)
Gene name: PLAT
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 49.74Å b: 73.94Å c: 201.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.203 0.276
Expression systems:
  • Escherichia coli
  • Komagataella phaffii CBS 7435