› 5boq

X-ray diffraction
1.7Å resolution

Human insulin with intra-chain chemical crosslink between modified B24 and B29

Released:
DOI: 10.2210/pdb5boq/pdb
PDB entry 5boq coloured by chain, front view.
PDB entry 5boq coloured by chain, side view.
PDB entry 5boq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Rational steering of insulin binding specificity by intra-chain chemical crosslinking.
Viková J, Collinsová M, Kletvíková E, Buděšínský M, Kaplan V, Žáková L, Veverka V, Hexnerová R, Tarazona Aviñó RJ, Straková J, Selicharová I, Vaněk V, Wright DW, Watson CJ, Turkenburg JP, Brzozowski AM, Jiráček J
Sci Rep 6 19431 (2016)
PMID: 26792393

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134210 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin A chain Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 21 amino acids
Theoretical weight: 2.38 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01308 (Residues: 90-110; Coverage: 24%)
Gene name: INS
Insulin B chain Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 30 amino acids
Theoretical weight: 3.41 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01308 (Residues: 25-54; Coverage: 35%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
2 modified residues:
Ligand HIX 4 x HIX
Ligand NLE 4 x NLE

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21212
Unit cell:
a: 55.438Å b: 56.865Å c: 60.012Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.196 0.232
Expression system: Not provided

Quick links

Citations

1 review citation

Insulin Receptor Isoforms in Physiology and Disease: An Updated View.
Belfiore et al. (2017)
6 other articles

1 mention without citation

Rational steering of insulin binding specificity by intra-chain chemical crosslinking.
Viková et al. (2016)