PDBe 5bnj

X-ray diffraction
2.64Å resolution

CDK8/CYCC IN COMPLEX WITH 8-{3-Chloro-5-[4-(1-methyl-1H-pyrazol-4-yl)-phenyl]-pyridin- 4-yl}-2,8-diaza-spiro[4.5]decan-1-one

Released:

Function and Biology Details

Reactions catalysed:
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cyclin-dependent kinase 8 Chain: A
Molecule details ›
Chain: A
Length: 405 amino acids
Theoretical weight: 46.99 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: NEW P49336 (Residues: 1-403; Coverage: 87%)
Gene name: CDK8
Sequence domains: Protein kinase domain
Structure domains:
Cyclin-C Chain: B
Molecule details ›
Chain: B
Length: 285 amino acids
Theoretical weight: 33.48 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: NEW P24863 (Residues: 1-283; Coverage: 100%)
Gene name: CCNC
Sequence domains:
Structure domains: Cyclin-like

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P212121
Unit cell:
a: 70.787Å b: 71.489Å c: 172.532Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.226 0.273
Expression system: Spodoptera frugiperda