PDBe 5b2t

X-ray diffraction
2.2Å resolution

Crystal structure of the Streptococcus pyogenes Cas9 VRER variant in complex with sgRNA and target DNA (TGCG PAM)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
CRISPR-associated endonuclease Cas9/Csn1 Chain: B
Molecule details ›
Chain: B
Length: 1372 amino acids
Theoretical weight: 159.08 KDa
Source organism: Streptococcus pyogenes serotype M1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99ZW2 (Residues: 1-1368; Coverage: 100%)
Gene names: SPy_1046, cas9, csn1
Sequence domains:
Target DNA Chain: C
Molecule details ›
Chain: C
Length: 28 nucleotides
Theoretical weight: 8.48 KDa
Source organism: Streptococcus pyogenes
Expression system: Not provided
Non-target DNA, DNA (5'-D(*TP*GP*CP*GP*AP*TP*TP*G)-3') Chain: D
Molecule details ›
Chain: D
Length: 8 nucleotides
Theoretical weight: 2.46 KDa
Source organism: Streptococcus pyogenes
Expression system: Not provided
Guide RNA Chain: A
Molecule details ›
Chain: A
Length: 81 nucleotides
Theoretical weight: 26.21 KDa
Source organism: Streptococcus pyogenes
Expression system: Not provided

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: C2
Unit cell:
a: 176.967Å b: 69.448Å c: 188.199Å
α: 90° β: 109.67° γ: 90°
R-values:
R R work R free
0.205 0.204 0.223
Expression systems:
  • Escherichia coli
  • Not provided