PDBe 5ajn

X-ray diffraction
1.67Å resolution

Crystal structure of the inactive form of GalNAc-T2 in complex with the glycopeptide MUC5AC-Cys13

Released:

Function and Biology Details

Reaction catalysed:
UDP-N-acetyl-alpha-D-galactosamine + [protein]-L-serine = UDP + [protein]-3-O-(N-acetyl-alpha-D-galactosaminyl)-L-serine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Polypeptide N-acetylgalactosaminyltransferase 2 Chain: A
Molecule details ›
Chain: A
Length: 571 amino acids
Theoretical weight: 64.82 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: NEW Q10471 (Residues: 1-571; Coverage: 100%)
Gene name: GALNT2
Sequence domains:
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A
Mucin-5AC Chain: P
Molecule details ›
Chain: P
Length: 16 amino acids
Theoretical weight: 1.48 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: NEW P98088 (Residues: 4566-4580; Coverage: 0%)
Gene names: MUC5, MUC5AC

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: I41
Unit cell:
a: 87.351Å b: 87.351Å c: 178.433Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.196 0.232
Expression system: Komagataella pastoris