› 4zrg

X-ray diffraction
2.7Å resolution

Visual arrestin mutant - R175E

Released:
DOI: 10.2210/pdb4zrg/pdb
PDB entry 4zrg coloured by chain, front view.
PDB entry 4zrg coloured by chain, side view.
PDB entry 4zrg coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Structural evidence for the role of polar core residue Arg175 in arrestin activation.
Granzin J, Stadler A, Cousin A, Schlesinger R, Batra-Safferling R
Sci Rep 5 15808 (2015)
PMID: 26510463

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139948 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-arrestin Chain: A
Molecule details ›
Chain: A
Length: 418 amino acids
Theoretical weight: 47.18 KDa
Source organism: Bos taurus
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P08168 (Residues: 1-404; Coverage: 100%)
Gene name: SAG
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CO2 5 x CO2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 66.373Å b: 72.314Å c: 79.405Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.194 0.241
Expression system: Saccharomyces cerevisiae

Quick links

Citations

5 review citations

Structure and dynamics of GPCR signaling complexes.
Hilger et al. (2018)
4 more

9 mentions without citation

Structural Basis of Arrestin-Dependent Signal Transduction.
Chen et al. (2018)
8 more