› 4zot

X-ray diffraction
1.4Å resolution

Crystal structure of BbKI, a disulfide-free plasma kallikrein inhibitor at 1.4 A resolution

Released:
DOI: 10.2210/pdb4zot/pdb
PDB entry 4zot coloured by chain, front view.
PDB entry 4zot coloured by chain, side view.
PDB entry 4zot coloured by chain, top view.
Source organism: Bauhinia bauhinioides
Primary publication:
Structure of BbKI, a disulfide-free plasma kallikrein inhibitor.
Zhou D, Hansen D, Shabalin IG, Gustchina A, Vieira DF, de Brito MV, Araújo AP, Oliva ML, Wlodawer A
Acta Crystallogr F Struct Biol Commun 71 1055-62 (2015)
PMID: 26249699

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160666 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kunitz-type serine protease inhibitor BbKI Chain: A
Molecule details ›
Chain: A
Length: 168 amino acids
Theoretical weight: 18.37 KDa
Source organism: Bauhinia bauhinioides
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P83052 (Residues: 1-164; Coverage: 100%)
Sequence domains: Trypsin and protease inhibitor
Structure domains: Trefoil (Acidic Fibroblast Growth Factor, subunit A)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 46.736Å b: 59.462Å c: 64.011Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.125 0.123 0.166
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Plant Kunitz Inhibitors and Their Interaction with Proteases: Current and Potential Pharmacological Targets.
Bonturi et al. (2022)
1 more

3 mentions without citation

Human plasma kallikrein: roles in coagulation, fibrinolysis, inflammation pathways, and beyond.
Motta et al. (2023)
2 more