› 4zm3

X-ray diffraction
2.27Å resolution

Crystal structure of PLP-Dependent 3-Aminobenzoate Synthase PctV wild-type

Released:
DOI: 10.2210/pdb4zm3/pdb
PDB entry 4zm3 coloured by chain, front view.
PDB entry 4zm3 coloured by chain, side view.
PDB entry 4zm3 coloured by chain, top view.
Source organism: Streptomyces pactum
Primary publication:
Mechanism-Based Trapping of the Quinonoid Intermediate by Using the K276R Mutant of PLP-Dependent 3-Aminobenzoate Synthase PctV in the Biosynthesis of Pactamycin.
Hirayama A, Miyanaga A, Kudo F, Eguchi T
Chembiochem 16 2484-90 (2015)
PMID: 26426567

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108779 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate-1-semialdehyde aminotransferase-like protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 464 amino acids
Theoretical weight: 49.7 KDa
Source organism: Streptomyces pactum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A8R0K5 (Residues: 1-444; Coverage: 100%)
Gene names: pctV, ptmT
Sequence domains: Aminotransferase class-III
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand PLP 4 x PLP
Ligand PGE 1 x PGE
Ligand PEG 3 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P21
Unit cell:
a: 58.99Å b: 170.83Å c: 74.57Å
α: 90° β: 98.33° γ: 90°
R-values:
R R work R free
0.182 0.18 0.222
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Pyridoxal phosphate-dependent reactions in the biosynthesis of natural products.
Du et al. (2019)
1 more