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Catalytic domain of Sst2 F403A mutant bound to ubiquitin

X-ray diffraction
1.72Å resolution
PDB entry 4zfr coloured by chain, front view.
PDB entry 4zfr coloured by chain, side view.
PDB entry 4zfr coloured by chain, top view.
Source organisms:
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: AMSH-like protease sst2 and Ubiquitin
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Multimeric state: hetero dimer
Accessible surface area: 11700.83 Å2
Buried surface area: 3006.03 Å2
Dissociation area: 1,264.15 Å2
Dissociation energy (ΔGdiss): 23.98 kcal/mol
Dissociation entropy (TΔSdiss): 10.98 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-143292

Macromolecules

AMSH-like protease sst2
Chain: A
Length: 197 amino acids
Theoretical weight: 21.91 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9P371 (Residues: 245-435; Coverage: 44%)
Gene names: SPAC19B12.10, sst2
Pfam: JAB1/Mov34/MPN/PAD-1 ubiquitin protease
InterPro:
CATH: Cytidine Deaminase, domain 2
AMSH-like protease sst2 in PDB entry 4zfr, assembly 1, front view.
AMSH-like protease sst2 in PDB entry 4zfr, assembly 1, side view.
AMSH-like protease sst2 in PDB entry 4zfr, assembly 1, top view.
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Ubiquitin
Chain: B
Length: 81 amino acids
Theoretical weight: 8.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CG47 (Residues: 153-228; Coverage: 33%)
Gene name: UBB
Pfam: Ubiquitin family
InterPro:
Ubiquitin in PDB entry 4zfr, assembly 1, front view.
Ubiquitin in PDB entry 4zfr, assembly 1, side view.
Ubiquitin in PDB entry 4zfr, assembly 1, top view.
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