› 4z7f

X-ray diffraction
3.19Å resolution

Crystal structure of FolT bound with folic acid

Released:
DOI: 10.2210/pdb4z7f/pdb
PDB entry 4z7f coloured by chain, front view.
PDB entry 4z7f coloured by chain, side view.
PDB entry 4z7f coloured by chain, top view.
Source organism: Enterococcus faecalis V583
Primary publication:
Structures of FolT in substrate-bound and substrate-released conformations reveal a gating mechanism for ECF transporters.
Zhao Q, Wang C, Wang C, Guo H, Bao Z, Zhang M, Zhang P
Nat Commun 6 7661 (2015)
PMID: 26198469

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182835 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Folate family ECF transporter S component Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 199 amino acids
Theoretical weight: 22.46 KDa
Source organism: Enterococcus faecalis V583
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q837A3 (Residues: 1-179; Coverage: 100%)
Gene name: EF_0940
Sequence domains: ECF transporter, substrate-specific component
Structure domains: Arp2/3 complex 21 kDa subunit ARPC3

Ligands and Environments

1 bound ligand:
Ligand FOL 6 x FOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P31
Unit cell:
a: 92.752Å b: 92.752Å c: 183.445Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.299 0.293 0.356
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Mechanism of Action of ABC Importers: Conservation, Divergence, and Physiological Adaptations.
Lewinson et al. (2017)
2 more

5 mentions without citation

Elevator-type mechanisms of membrane transport.
Garaeva et al. (2020)
4 more