PDBe 4ymu

X-ray diffraction
2.5Å resolution

Crystal structure of an amino acid ABC transporter complex with arginines and ATPs

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ABC-type amino acid transport system, permease component Chains: C, D
Molecule details ›
Chains: C, D
Length: 220 amino acids
Theoretical weight: 24.25 KDa
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis MB4
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8RCC3 (Residues: 1-220; Coverage: 100%)
Gene names: ArtM, TTE0513
Sequence domains: Binding-protein-dependent transport system inner membrane component
Structure domains: MetI-like
ABC-type polar amino acid transport system, ATPase component Chains: A, J
Molecule details ›
Chains: A, J
Length: 240 amino acids
Theoretical weight: 26.82 KDa
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis MB4
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8RCC2 (Residues: 1-240; Coverage: 100%)
Gene names: GlnQ, TTE0514
Sequence domains: ABC transporter
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2221
Unit cell:
a: 96.765Å b: 114.098Å c: 298.636Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.224 0.26
Expression system: Escherichia coli