› 4ykf

X-ray diffraction
2.5Å resolution

Crystal Structure of the Alkylhydroperoxide Reductase subunit F (AhpF) with NADH from Escherichia coli

Released:
DOI: 10.2210/pdb4ykf/pdb
PDB entry 4ykf coloured by chain, front view.
PDB entry 4ykf coloured by chain, side view.
PDB entry 4ykf coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Crystallographic and solution studies of NAD(+)- and NADH-bound alkylhydroperoxide reductase subunit F (AhpF) from Escherichia coli provide insight into sequential enzymatic steps.
Kamariah N, Manimekalai MS, Nartey W, Eisenhaber F, Eisenhaber B, Grüber G
Biochim Biophys Acta 1847 1139-52 (2015)
PMID: 26092085

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152889 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alkyl hydroperoxide reductase subunit F Chain: A
Molecule details ›
Chain: A
Length: 521 amino acids
Theoretical weight: 56.24 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P35340 (Residues: 1-521; Coverage: 100%)
Gene names: JW0599, ahpF, b0606
Sequence domains:
Structure domains:

Ligands and Environments

6 bound ligands:
Ligand FAD 1 x FAD
Ligand NAI 1 x NAI
Ligand CD 1 x CD
Ligand SO4 10 x SO4
Ligand GOL 3 x GOL
Ligand PEG 3 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: C2
Unit cell:
a: 107.09Å b: 59.39Å c: 121.35Å
α: 90° β: 111.34° γ: 90°
R-values:
R R work R free
0.228 0.225 0.288
Expression system: Escherichia coli

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Citations

10 citation in other articles

Transition steps in peroxide reduction and a molecular switch for peroxide robustness of prokaryotic peroxiredoxins.
Kamariah et al. (2016)
9 more