› 4yj6

X-ray diffraction
1.7Å resolution

The Crystal Structure of a Bacterial Aryl Acylamidase Belonging to the Amidase signature (AS) enzymes family

Released:
DOI: 10.2210/pdb4yj6/pdb
PDB entry 4yj6 coloured by chain, front view.
PDB entry 4yj6 coloured by chain, side view.
PDB entry 4yj6 coloured by chain, top view.
Source organism: bacterium CSBL00001
Primary publication:
Crystal structure analysis of a bacterial aryl acylamidase belonging to the amidase signature enzyme family.
Lee S, Park EH, Ko HJ, Bang WG, Kim HY, Kim KH, Choi IG
Biochem Biophys Res Commun 467 268-74 (2015)
PMID: 26454172

Function and Biology Details

Reaction catalysed: 
An anilide + H(2)O = a carboxylate + aniline
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111227 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Amidase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 501 amino acids
Theoretical weight: 54.76 KDa
Source organism: bacterium CSBL00001
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C3UWD1 (Residues: 1-495; Coverage: 100%)
Sequence domains: Amidase
Structure domains: Amidase signature (AS) domain

Ligands and Environments

1 bound ligand:
Ligand PO4 2 x PO4
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P41212
Unit cell:
a: 75.76Å b: 75.76Å c: 214Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.155 0.155 0.168
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Human Serine Racemase: Key Residues/Active Site Motifs and Their Relation to Enzyme Function.
Graham et al. (2019)
6 other articles

2 mentions without citation

Structural analysis of a plant fatty acid amide hydrolase provides insights into the evolutionary diversity of bioactive acylethanolamides.
Aziz et al. (2019)
1 more