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Crystallographic snapshots of the EF-hand protein MCFD2 complexed with the intracellular lectin ERGIC-53 involved in glycoprotein transport.

Satoh T, Nishio M, Suzuki K, Yagi-Utsumi M, Kamiya Y, Mizushima T, Kato K
Acta Crystallogr F Struct Biol Commun 76 216-221 (2020)
Related entries: 4ygb, 4ygd, 4yge

Cited: 5 times
EuropePMC logo PMID: 32356523

Abstract

The transmembrane intracellular lectin ER-Golgi intermediate compartment protein 53 (ERGIC-53) and the soluble EF-hand multiple coagulation factor deficiency protein 2 (MCFD2) form a complex that functions as a cargo receptor, trafficking various glycoproteins between the endoplasmic reticulum (ER) and the Golgi apparatus. It has been demonstrated that the carbohydrate-recognition domain (CRD) of ERGIC-53 (ERGIC-53CRD) interacts with N-linked glycans on cargo glycoproteins, whereas MCFD2 recognizes polypeptide segments of cargo glycoproteins. Crystal structures of ERGIC-53CRD complexed with MCFD2 and mannosyl oligosaccharides have revealed protein-protein and protein-sugar binding modes. In contrast, the polypeptide-recognition mechanism of MCFD2 remains largely unknown. Here, a 1.60 Å resolution crystal structure of the ERGIC-53CRD-MCFD2 complex is reported, along with three other crystal forms. Comparison of these structures with those previously reported reveal that MCFD2, but not ERGIC-53-CRD, exhibits significant conformational plasticity that may be relevant to its accommodation of various polypeptide ligands.

Articles - 4ygc mentioned but not cited (1)

  1. Crystallographic snapshots of the EF-hand protein MCFD2 complexed with the intracellular lectin ERGIC-53 involved in glycoprotein transport. Satoh T, Nishio M, Suzuki K, Yagi-Utsumi M, Kamiya Y, Mizushima T, Kato K. Acta Crystallogr F Struct Biol Commun 76 216-221 (2020)


Reviews citing this publication (2)

  1. Glycans in autophagy, endocytosis and lysosomal functions. Reggiori F, Gabius HJ, Aureli M, Römer W, Sonnino S, Eskelinen EL. Glycoconj J 38 625-647 (2021)
  2. Mammalian cargo receptors for endoplasmic reticulum-to-Golgi transport: mechanisms and interactions. Zhang Y, Srivastava V, Zhang B. Biochem Soc Trans 51 971-981 (2023)

Articles citing this publication (2)

  1. LMAN1-MCFD2 complex is a cargo receptor for the ER-Golgi transport of α1-antitrypsin. Zhang Y, Zhu M, Zheng C, Wei W, Emmer BT, Zhang B. Biochem J 479 839-855 (2022)
  2. Separate roles of LMAN1 and MCFD2 in ER-to-Golgi trafficking of FV and FVIII. Zhang Y, Liu Z, Zhang B. Blood Adv 7 1286-1296 (2023)


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