4yga

X-ray diffraction
2.94Å resolution

CDPK1, from Toxoplasma gondii, bound to inhibitory VHH-1B7

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-212706 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calmodulin-domain protein kinase 1 Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 516 amino acids
Theoretical weight: 58.54 KDa
Source organism: Toxoplasma gondii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9BJF5 (Residues: 1-507; Coverage: 100%)
Gene name: CDPK1
Sequence domains:
Structure domains:
VHH-1B7 Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 141 amino acids
Theoretical weight: 15.44 KDa
Source organism: Vicugna pacos
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P1
Unit cell:
a: 76.92Å b: 91.067Å c: 106.641Å
α: 88.71° β: 108.27° γ: 90.26°
R-values:
R R work R free
0.227 0.226 0.262
Expression system: Escherichia coli BL21(DE3)