› 4xuz

X-ray diffraction
1.5Å resolution

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197432 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase Chain: A
Molecule details ›
Chain: A
Length: 265 amino acids
Theoretical weight: 28.28 KDa
Source organism: Klebsiella pneumoniae subsp. pneumoniae
Expression system: Escherichia coli
UniProt:
  • Canonical: S5LVN3 (Residues: 29-291; Coverage: 100%)
Gene names: blaCTX-M, blaCTX-M-15
Sequence domains:
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

4 bound ligands:
Ligand CL 1 x CL
Ligand CIT 1 x CIT
Ligand EDO 9 x EDO
Ligand 4D6 1 x 4D6
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P212121
Unit cell:
a: 44.84Å b: 45.81Å c: 116.85Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.133 0.132 0.147
Expression system: Escherichia coli

Quick links

Citations

78 review citations

β-Lactams and β-Lactamase Inhibitors: An Overview.
Bush et al. (2016)
77 more

5 mentions without citation

β-Lactamases and β-Lactamase Inhibitors in the 21st Century.
Tooke et al. (2019)
4 more