› 4xux

X-ray diffraction
1.75Å resolution

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138639 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase Chain: A
Molecule details ›
Chain: A
Length: 363 amino acids
Theoretical weight: 39.41 KDa
Source organism: Enterobacter cloacae
Expression system: Escherichia coli
UniProt:
  • Canonical: P05364 (Residues: 21-381; Coverage: 100%)
Gene name: ampC
Sequence domains: Beta-lactamase
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

3 bound ligands:
Ligand 4D6 1 x 4D6
Ligand EDO 2 x EDO
Ligand PG0 1 x PG0
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Unit cell:
a: 62.56Å b: 69.6Å c: 76.33Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.154 0.186
Expression system: Escherichia coli

Quick links

Citations

80 review citations

β-Lactams and β-Lactamase Inhibitors: An Overview.
Bush et al. (2016)
79 more

5 mentions without citation

Targeting Antibiotic Resistance.
Chellat et al. (2016)
4 more