› 4xr0

X-ray diffraction
2.8Å resolution

Escherichia Coli Replication Terminator Protein (Tus) Complexed With DNA- G/T mismatch.

Released:
DOI: 10.2210/pdb4xr0/pdb
PDB entry 4xr0 coloured by chain, front view.
PDB entry 4xr0 coloured by chain, side view.
PDB entry 4xr0 coloured by chain, top view.
Source organisms:
Primary publication:
Replisome speed determines the efficiency of the Tus-Ter replication termination barrier.
Elshenawy MM, Jergic S, Xu ZQ, Sobhy MA, Takahashi M, Oakley AJ, Dixon NE, Hamdan SM
Nature 525 394-8 (2015)
PMID: 26322585

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118281 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA replication terminus site-binding protein Chain: A
Molecule details ›
Chain: A
Length: 316 amino acids
Theoretical weight: 36.8 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P16525 (Residues: 1-309; Coverage: 100%)
Gene names: JW1602, b1610, tau, tus
Sequence domains: DNA replication terminus site-binding protein (Ter protein)
Structure domains:
DNA (5'-D(*AP*GP*TP*TP*AP*CP*AP*AP*CP*AP*TP*AP*GP*T)-3') Chain: B
Molecule details ›
Chain: B
Length: 16 nucleotides
Theoretical weight: 4.9 KDa
Source organism: Escherichia coli
Expression system: Not provided
DNA (5'-D(*AP*TP*TP*AP*TP*GP*TP*TP*GP*TP*AP*AP*CP*TP*A)-3') Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 4.9 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand IOD 5 x IOD
Ligand MPD 1 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P41212
Unit cell:
a: 64.523Å b: 64.523Å c: 248.335Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.218 0.296
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

10 review citations

Xer Site Specific Recombination: Double and Single Recombinase Systems.
Castillo et al. (2017)
9 more

2 mentions without citation

Autonomous aggregation suppression by acidic residues explains why chaperones favour basic residues.
Houben et al. (2020)
1 more