› 4xnj

X-ray diffraction
2.3Å resolution

X-ray structure of PepTst2

Released:
DOI: 10.2210/pdb4xnj/pdb
PDB entry 4xnj coloured by chain, front view.
PDB entry 4xnj coloured by chain, side view.
PDB entry 4xnj coloured by chain, top view.
Source organism: Streptococcus thermophilus LMG 18311
Primary publication:
In meso in situ serial X-ray crystallography of soluble and membrane proteins.
Huang CY, Olieric V, Ma P, Panepucci E, Diederichs K, Wang M, Caffrey M
Acta Crystallogr D Biol Crystallogr 71 1238-56 (2015)
PMID: 26057665

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177587 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Major facilitator superfamily (MFS) profile domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 483 amino acids
Theoretical weight: 52.78 KDa
Source organism: Streptococcus thermophilus LMG 18311
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5M4H8 (Residues: 1-483; Coverage: 100%)
Gene names: dtpT, stu0970
Sequence domains: POT family
Structure domains: MFS general substrate transporter like domains

Ligands and Environments

2 bound ligands:
Ligand 78M 13 x 78M
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: C2221
Unit cell:
a: 102.88Å b: 110.16Å c: 110.96Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.203 0.251
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Understanding transport by the major facilitator superfamily (MFS): structures pave the way.
Quistgaard et al. (2016)
2 more

5 mentions without citation

Recent advances in understanding prodrug transport through the SLC15 family of proton-coupled transporters.
Minhas et al. (2020)
4 more