› 4xco

X-ray diffraction
2.9Å resolution

Signal-sequence induced conformational changes in the signal recognition particle

Released:
DOI: 10.2210/pdb4xco/pdb
PDB entry 4xco coloured by chain, front view.
PDB entry 4xco coloured by chain, side view.
PDB entry 4xco coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii
Primary publication:
Signal-sequence induced conformational changes in the signal recognition particle.
Hainzl T, Sauer-Eriksson AE
Nat Commun 6 7163 (2015)
PMID: 26051119

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176436 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
Signal recognition particle 19 kDa protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 87 amino acids
Theoretical weight: 10.38 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q58440 (Residues: 1-87; Coverage: 100%)
Gene names: MJ1034, srp19
Sequence domains: SRP19 protein
Structure domains: Signal recognition particle, SRP19-like subunit
Signal recognition particle 54 kDa protein Chains: C, D
Molecule details ›
Chains: C, D
Length: 173 amino acids
Theoretical weight: 19.21 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Lactococcus lactis
UniProt:
  • Canonical: Q57565 (Residues: 303-451; Coverage: 33%)
Gene names: MJ0101, srp54
Sequence domains: Signal peptide binding domain
Structure domains: Signal recognition particle, SRP54 subunit, M-domain
RNA Chains: E, M
Molecule details ›
Chains: E, M
Length: 96 nucleotides
Theoretical weight: 31.16 KDa
Sequence domains: Bacterial small signal recognition particle RNA

Ligands and Environments

2 bound ligands:
Ligand MG 14 x MG
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Unit cell:
a: 91.227Å b: 112.971Å c: 121.204Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.209 0.258
Expression systems:
  • Escherichia coli BL21(DE3)
  • Lactococcus lactis

Quick links

Citations

6 review citations

Protein export through the bacterial Sec pathway.
Tsirigotaki et al. (2017)
5 more

3 mentions without citation

A Novel SRP Recognition Sequence in the Homeostatic Control Region of Heat Shock Transcription Factor σ32.
Miyazaki et al. (2016)
2 more