› 4x4k

X-ray diffraction
2.3Å resolution

Structure of laccase from Botrytis aclada with full copper content

Released:
DOI: 10.2210/pdb4x4k/pdb
PDB entry 4x4k coloured by chain, front view.
PDB entry 4x4k coloured by chain, side view.
PDB entry 4x4k coloured by chain, top view.
Source organism: Botrytis aclada
Primary publication:
Incorporation of copper ions into crystals of T2 copper-depleted laccase from Botrytis aclada.
Osipov EM, Polyakov KM, Tikhonova TV, Kittl R, Dorovatovskii PV, Shleev SV, Popov VO, Ludwig R
Acta Crystallogr F Struct Biol Commun 71 1465-9 (2015)
PMID: 26625287

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124899 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Laccase Chain: A
Molecule details ›
Chain: A
Length: 580 amino acids
Theoretical weight: 63.67 KDa
Source organism: Botrytis aclada
Expression system: Komagataella pastoris
UniProt:
  • Canonical: H8ZRU2 (Residues: 1-580; Coverage: 100%)
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, MAN, BMA
Carbohydrate polymer
4 bound ligands:
Ligand CU 5 x CU
Ligand NAG 5 x NAG
Ligand MAN 1 x MAN
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: KURCHATOV SNC BEAMLINE K4.4
Spacegroup: C2
Unit cell:
a: 69.4Å b: 113.1Å c: 79.6Å
α: 90° β: 109° γ: 90°
R-values:
R R work R free
0.184 0.181 0.233
Expression system: Komagataella pastoris

Quick links

Citations

6 citation in other articles

Structural study of the X-ray-induced enzymatic reduction of molecular oxygen to water by Steccherinum murashkinskyi laccase: insights into the reaction mechanism.
Polyakov et al. (2017)
5 more

1 mention without citation

Incorporation of copper ions into crystals of T2 copper-depleted laccase from Botrytis aclada.
Osipov et al. (2015)