4wzy

X-ray diffraction
1.71Å resolution

Structure of mycobacterial maltokinase, the missing link in the essential GlgE-pathway (ATP complex)

Released:

Function and Biology Details

Reaction catalysed:
ATP + maltose = ADP + alpha-maltose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106707 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Maltokinase Chain: A
Molecule details ›
Chain: A
Length: 454 amino acids
Theoretical weight: 49.75 KDa
Source organism: Mycolicibacterium vanbaalenii PYR-1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A1TH50 (Residues: 1-441; Coverage: 100%)
Gene names: Mvan_5735, mak
Sequence domains: Maltokinase N-terminal cap domain
Structure domains: Aminoglycoside 3'-phosphotransferase; Chain: A, domain 2

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P2221
Unit cell:
a: 62.991Å b: 73.767Å c: 107.426Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.177 0.205
Expression system: Escherichia coli BL21(DE3)