› 4wvz

X-ray diffraction
2.09Å resolution

Crystal structure of artificial crosslinked thiol dioxygenase G95C variant from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb4wvz/pdb
PDB entry 4wvz coloured by chain, front view.
PDB entry 4wvz coloured by chain, side view.
PDB entry 4wvz coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Substrate and pH-Dependent Kinetic Profile of 3-Mercaptopropionate Dioxygenase from Pseudomonas aeruginosa.
Fellner M, Aloi S, Tchesnokov EP, Wilbanks SM, Jameson GN
Biochemistry 55 1362-71 (2016)
PMID: 26878277

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102983 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thiol dioxygenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 211 amino acids
Theoretical weight: 23.82 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140UH61 (Residues: 1-201; Coverage: 95%)
Gene name: Q034_01739
Sequence domains: Cysteine dioxygenase type I
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand FE2 4 x FE2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P41212
Unit cell:
a: 66.543Å b: 66.543Å c: 376.852Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.175 0.218
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Carbon-fluorine bond cleavage mediated by metalloenzymes.
Wang et al. (2020)
9 other articles