Structure analysis

Crystal structure of the whole ribosomal complex.

X-ray diffraction
5.9Å resolution
Assembly composition:
hetero 56-mer (preferred)
Entry contents: 50 distinct polypeptide molecules
6 distinct RNA molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero 56-mer
Accessible surface area: 811499.68 Å2
Buried surface area: 228016.18 Å2
Dissociation area: 542.64 Å2
Dissociation energy (ΔGdiss): -3.46 kcal/mol
Dissociation entropy (TΔSdiss): 13.63 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-215826

Macromolecules

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Chain: AH
Length: 138 amino acids
Theoretical weight: 15.87 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P0DOY9 (Residues: 1-138; Coverage: 100%)
Gene names: TTHA1678, rpsH
Pfam: Ribosomal protein S8
InterPro:

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Chain: AL
Length: 135 amino acids
Theoretical weight: 14.92 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SHN3 (Residues: 1-132; Coverage: 100%)
Gene names: TTHA1697, rpsL
Pfam: Ribosomal protein S12/S23
InterPro:

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Chain: AO
Length: 89 amino acids
Theoretical weight: 10.58 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SJ76 (Residues: 1-89; Coverage: 100%)
Gene names: TTHA1138, rpsO
Pfam: Ribosomal protein S15
InterPro:

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Chain: AP
Length: 88 amino acids
Theoretical weight: 10.41 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SJH3 (Residues: 1-88; Coverage: 100%)
Gene names: TTHA1035, rpsP
Pfam: Ribosomal protein S16
InterPro:

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Chain: AQ
Length: 105 amino acids
Theoretical weight: 12.32 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P0DOY7 (Residues: 1-105; Coverage: 100%)
Gene names: TTHA1683, rpsQ
Pfam: Ribosomal protein S17
InterPro:

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Chain: AR
Length: 88 amino acids
Theoretical weight: 10.24 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SLQ0 (Residues: 1-88; Coverage: 100%)
Gene names: TTHA0243, rpsR
Pfam: Ribosomal protein S18
InterPro:

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Chain: AT
Length: 106 amino acids
Theoretical weight: 11.72 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P80380 (Residues: 1-106; Coverage: 100%)
Gene names: TTHA1397, rps20, rpsT
Pfam: Ribosomal protein S20
InterPro:

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Chain: AU
Length: 27 amino acids
Theoretical weight: 3.35 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SIH3 (Residues: 1-27; Coverage: 100%)
Gene names: TTHA1396, rpsU
Pfam: 30S ribosomal protein Thx
InterPro:

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Chain: AY
Length: 354 amino acids
Theoretical weight: 40.15 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: P96077 (Residues: 1-354; Coverage: 100%)
Gene names: TTHA1940, prfA
Pfam:
InterPro:

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Chain: BO
Length: 122 amino acids
Theoretical weight: 13.37 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q5L413 (Residues: 1-122; Coverage: 100%)
Gene names: GK0116, rplN
Pfam: Ribosomal protein L14p/L23e
InterPro:

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Chain: BS
Length: 186 amino acids
Theoretical weight: 20.51 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: P14123 (Residues: 2-187; Coverage: 100%)
Gene names: rpl18, rrnAC1593
Pfam: Ribosomal large subunit proteins 60S L5, and 50S L18
InterPro: Large ribosomal subunit protein uL18 eukaryotic/archaeal

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Chain: BR
Length: 118 amino acids
Theoretical weight: 13.75 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q9Z9H5 (Residues: 1-118; Coverage: 100%)
Gene names: TTHA1663, rpl17, rplQ
Pfam: Ribosomal protein L17
InterPro:

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Chain: BU
Length: 118 amino acids
Theoretical weight: 13.99 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q9RSW7 (Residues: 1-118; Coverage: 100%)
Gene names: DR_2004, rplT
Pfam: Ribosomal protein L20
InterPro:

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Chain: BV
Length: 100 amino acids
Theoretical weight: 11.17 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q9RY64 (Residues: 1-100; Coverage: 100%)
Gene names: DR_0086, rplU
Pfam: Ribosomal prokaryotic L21 protein
InterPro:

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Chain: B2
Length: 70 amino acids
Theoretical weight: 7.76 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: P10971 (Residues: 2-71; Coverage: 99%)
Gene names: rpl29, rrnAC1604
Pfam: Ribosomal L29 protein
InterPro:

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Chain: B0
Length: 91 amino acids
Theoretical weight: 9.61 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q9RY65 (Residues: 1-91; Coverage: 100%)
Gene names: DR_0085, rpmA
Pfam: Ribosomal L27 protein
InterPro:

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Chain: B5
Length: 60 amino acids
Theoretical weight: 6.81 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: P49228 (Residues: 1-60; Coverage: 100%)
Gene names: DR_2366, rpmF
Pfam: Ribosomal L32p protein family
InterPro:

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Chain: B6
Length: 82 amino acids
Theoretical weight: 9.29 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q9RSS4 (Residues: 1-55; Coverage: 100%)
Gene names: DR_2049, rpmG
Pfam: Ribosomal protein L33
InterPro:

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Chain: B7
Length: 47 amino acids
Theoretical weight: 5.63 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q9RSH2 (Residues: 1-47; Coverage: 100%)
Gene names: DR_2152, rpmH
Pfam: Ribosomal protein L34
InterPro:

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Chain: B9
Length: 36 amino acids
Theoretical weight: 4.27 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q9RSK0 (Residues: 1-36; Coverage: 97%)
Gene names: DR_2124, rpmJ
Pfam: Ribosomal protein L36
InterPro:

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Name: 16S ribosomal RNA
Representative chains: AA
Length: 1522 nucleotides
Theoretical weight: 493.96 KDa
Rfam: Bacterial small subunit ribosomal RNA

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Name: P-site tRNA (Phe)
Representative chains: AV
Length: 76 nucleotides
Theoretical weight: 24.52 KDa
Rfam: tRNA

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Name: E-site tRNA (Phe)
Representative chains: AW
Length: 76 nucleotides
Theoretical weight: 24.74 KDa
Rfam: tRNA

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Name: 5'-R(*AP*UP*GP*UP*UP*CP*UP*AP*GP*UP*AP*CP*AP*AP*UP*AP*AP*U)-3'
Representative chains: AX
Length: 18 nucleotides
Theoretical weight: 5.7 KDa

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Name: 5S ribosomal RNA
Representative chains: BB
Length: 123 nucleotides
Theoretical weight: 39.85 KDa
Rfam: 5S ribosomal RNA

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Name: 23S ribosomal RNA
Representative chains: BA
Length: 2916 nucleotides
Theoretical weight: 948.28 KDa
Rfam: Bacterial large subunit ribosomal RNA

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