› 4v0o

X-ray diffraction
3.35Å resolution

Crystal structure of BBS1N in complex with ARL6DN, soaked with lead

Released:
DOI: 10.2210/pdb4v0o/pdb
PDB entry 4v0o coloured by chain, front view.
PDB entry 4v0o coloured by chain, side view.
PDB entry 4v0o coloured by chain, top view.
Source organism: Chlamydomonas reinhardtii
Primary publication:
Structural basis for membrane targeting of the BBSome by ARL6.
Mourão A, Nager AR, Nachury MV, Lorentzen E
Nat Struct Mol Biol 21 1035-41 (2014)
PMID: 25402481

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108701 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ARF-LIKE SMALL GTPASE Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 169 amino acids
Theoretical weight: 18.81 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Trichoplusia ni
UniProt:
  • Canonical: A8JF99 (Residues: 16-180; Coverage: 92%)
Gene name: CHLRE_16g664500v5
Sequence domains: ADP-ribosylation factor family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
BARDET-BIEDL SYNDROME 1 PROTEIN Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 425 amino acids
Theoretical weight: 46.37 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Trichoplusia ni

Ligands and Environments

3 bound ligands:
Ligand GTP 4 x GTP
Ligand MG 4 x MG
Ligand PB 18 x PB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P3121
Unit cell:
a: 123.698Å b: 123.698Å c: 439.022Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.224 0.221 0.268
Expression system: Trichoplusia ni

Quick links

Citations

17 review citations

IFT-Cargo Interactions and Protein Transport in Cilia.
Lechtreck KF. (2015)
16 more

2 mentions without citation

Structural basis for membrane targeting of the BBSome by ARL6.
Mourão et al. (2014)
1 more