› 4uun

X-ray diffraction
1.78Å resolution

Trichomonas vaginalis lactate dehydrogenase in complex with NADH

Released:
DOI: 10.2210/pdb4uun/pdb
PDB entry 4uun coloured by chain, front view.
PDB entry 4uun coloured by chain, side view.
PDB entry 4uun coloured by chain, top view.
Source organism: Trichomonas vaginalis
Primary publication:
Gradual neofunctionalization in the convergent evolution of trichomonad lactate and malate dehydrogenases.
Steindel PA, Chen EH, Wirth JD, Theobald DL
Protein Sci 25 1319-31 (2016)
PMID: 26889885

Function and Biology Details

Reaction catalysed: 
(S)-lactate + NAD(+) = pyruvate + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132147 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-lactate dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 341 amino acids
Theoretical weight: 37.95 KDa
Source organism: Trichomonas vaginalis
Expression system: Escherichia coli
UniProt:
  • Canonical: O96445 (Residues: 1-333; Coverage: 100%)
Gene name: LDH1
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAI 2 x NAI
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 82.57Å b: 59.61Å c: 86.49Å
α: 90° β: 116.18° γ: 90°
R-values:
R R work R free
0.154 0.154 0.19
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Evolution of protein specificity: insights from ancestral protein reconstruction.
Siddiq et al. (2017)
1 more

3 mentions without citation

Comparative Protein Structure Modeling Using MODELLER.
Webb et al. (2016)
2 more