› 4uhp

X-ray diffraction
2Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175947 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
HNH nuclease domain-containing protein Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 136 amino acids
Theoretical weight: 15.15 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q51502 (Residues: 642-777; Coverage: 18%)
Sequence domains: Colicin/Pyocin-S2, DNase domain
Structure domains: Colicin/pyocin, DNase domain
Small component of pyocin AP41 Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 98 amino acids
Theoretical weight: 11.37 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q51503 (Residues: 1-90; Coverage: 100%)
Sequence domains: Colicin immunity protein / pyocin immunity protein
Structure domains: Colicin E immunity protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P1
Unit cell:
a: 36.604Å b: 75.627Å c: 83.171Å
α: 79.19° β: 78.05° γ: 77°
R-values:
R R work R free
0.188 0.186 0.235
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Lectin-Like Bacteriocins.
Ghequire et al. (2018)
13 other articles

10 mentions without citation

Structures of the Ultra-High-Affinity Protein-Protein Complexes of Pyocins S2 and AP41 and Their Cognate Immunity Proteins from Pseudomonas aeruginosa.
Joshi et al. (2015)
9 more